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基于氧化石墨烯與金屬離子的邏輯模型設(shè)計(jì)與可控性驗(yàn)證

王璐慧,  王越,  錢夢瑤,  董亞非

王璐慧, 王越, 錢夢瑤, 董亞非. 基于氧化石墨烯與金屬離子的邏輯模型設(shè)計(jì)與可控性驗(yàn)證[J]. 電子與信息學(xué)報(bào), 2020, 42(6): 1410-1419. doi: 10.11999/JEIT190872
引用本文: 王璐慧, 王越, 錢夢瑤, 董亞非. 基于氧化石墨烯與金屬離子的邏輯模型設(shè)計(jì)與可控性驗(yàn)證[J]. 電子與信息學(xué)報(bào), 2020, 42(6): 1410-1419. doi: 10.11999/JEIT190872
Luhui WANG, Yue WANG, Mengyao QIAN, Yafei DONG. Logical Model Design and Controllability Verification Based on Graphene Oxide and Metal Ions[J]. Journal of Electronics & Information Technology, 2020, 42(6): 1410-1419. doi: 10.11999/JEIT190872
Citation: Luhui WANG, Yue WANG, Mengyao QIAN, Yafei DONG. Logical Model Design and Controllability Verification Based on Graphene Oxide and Metal Ions[J]. Journal of Electronics & Information Technology, 2020, 42(6): 1410-1419. doi: 10.11999/JEIT190872

基于氧化石墨烯與金屬離子的邏輯模型設(shè)計(jì)與可控性驗(yàn)證

doi: 10.11999/JEIT190872 cstr: 32379.14.JEIT190872
  • 1.

    陜西師范大學(xué)生命科學(xué)學(xué)院 西安 710119

  • 2.

    陜西師范大學(xué)計(jì)算機(jī)科學(xué)學(xué)院 西安 710119

基金項(xiàng)目: 國家自然科學(xué)基金(61572302)，陜西省自然科學(xué)基礎(chǔ)研究計(jì)劃(2020JM-298)，陜西師范大學(xué)博士研究生自由探索項(xiàng)目(2019TS075)
詳細(xì)信息
    作者簡介:

    王璐慧：女，1993年，博士生，研究方向?yàn)樯镄畔W(xué)、DNA計(jì)算、生物傳感器

    王越：女，1994年，碩士生，研究方向?yàn)镈NA計(jì)算、合成生物學(xué)

    錢夢瑤：女，1995年，碩士生，研究方向?yàn)镈NA計(jì)算、生物傳感器

    董亞非：男，1963年，教授，研究方向?yàn)樯镄畔W(xué)、DNA計(jì)算、生物傳感器、合成生物學(xué)

    通訊作者:

    董亞非　dongyf@snnu.edu.cn

  • 中圖分類號: TP301

Logical Model Design and Controllability Verification Based on Graphene Oxide and Metal Ions

  • 1.

    School of Life Science, Shaanxi Normal University, Xi’an 710119, China

  • 2.

    School of Computing Technology, Shaanxi Normal University, Xi’an 710119, China

Funds: The National Natural Science Foundation of China (61572302), The Basic Research Plan of Natural Science of Shaanxi Province(2020JM-298), The Free Exploration Project for Doctoral Students of Shaanxi Normal University (2019TS075)
  • 摘要: 作為20世紀(jì)末誕生的新型學(xué)科，生物計(jì)算現(xiàn)已成為前沿科學(xué)研究的熱點(diǎn)。與電子計(jì)算機(jī)相似，生物計(jì)算機(jī)的構(gòu)建需要多種分子邏輯門，而將氧化石墨烯(GO)、重金屬離子等具有生化特性的物質(zhì)引入分子邏輯門的設(shè)計(jì)中，有望為研究提出新思路。此外，分子邏輯門最終應(yīng)在生物實(shí)驗(yàn)層面上實(shí)現(xiàn)，這就需要對生物實(shí)驗(yàn)各個(gè)條件的可控性及可控范圍進(jìn)行研究?；谶@樣的想法，該文以氧化石墨烯和金屬離子為基礎(chǔ)設(shè)計(jì)了多個(gè)邏輯門，通過仿真實(shí)驗(yàn)、電泳實(shí)驗(yàn)、正交實(shí)驗(yàn)、熒光實(shí)驗(yàn)等驗(yàn)證可行性的同時(shí)，對實(shí)驗(yàn)的可控性及可控范圍有進(jìn)一步研究，一方面證明了所設(shè)計(jì)邏輯門可行性，另一方面也發(fā)現(xiàn)其有運(yùn)用于實(shí)際樣品檢測的能力。
    Abstract: As a new subject born at the end of the 20th century, biological computing has become a hot spot of frontier scientific research. Similar to the electronic computer, the construction of biological computer needs a variety of molecular logic gates, and the introduction of Graphene Oxide(GO), heavy metal ion heavy metal particles and other substances with biochemical characteristics into the design of molecular logic gates is expected to put forward new ideas for research. In addition, molecular logic gates should be realized at the level of biological experiments, which requires the study of controllability and controllable range of various conditions of biological experiments. Based on this idea, several logic gates are designed based on graphene oxide and metal ions. The feasibility is verified by simulation experiment, electrophoresis experiment, orthogonal experiment, fluorescence experiment, etc. At the same time, the controllability and controllable range of the experiment are further researched. On the one hand, the feasibility of the designed logic gate is proved, on the other hand, it be found that can be applied to actual samples.
    • HTML全文

  • 圖  1  YES門與AND門原理及真值表

    圖  2  YES門及AND門仿真結(jié)果

    圖  3  YES門PAGE電泳結(jié)果

    圖  4  AND門瓊脂糖電泳結(jié)果

    圖  5  實(shí)驗(yàn)條件可控性驗(yàn)證及優(yōu)化

    圖  6  目標(biāo)響應(yīng)范圍及線性相關(guān)性

    圖  7  基于金屬離子的多功能模型原理及真值表

    圖  8  基于金屬離子的多功能模型仿真結(jié)果

    圖  9  實(shí)驗(yàn)條件可控性驗(yàn)證

    圖  10  汞離子與銀離子分別存在時(shí)目標(biāo)響應(yīng)范圍及線性相關(guān)性

    圖  11  基于氧化石墨烯與金屬離子的3輸入邏輯門原理、真值表及電泳結(jié)果

    表  1  DNA鏈序列

    鏈名稱鏈序列(5’-3’)
    TargetGACATTCATCACGCTCAATCACTACTT
    H1TAMRA - AAGTAGTGATTGAGCGTGATGAATGTC
    HH1AAGTAGTGATTGAGCGTGATGAATGTCACTACTTCAACTCGCATTCATCACGCTCAATC
    HH2TAMRA - TGATGAATGCGAGTTGAAGTAGTGACATTCATCACGCTCAATCACTACTTCAACTCGCA
    下載: 導(dǎo)出CSV

    表  2  DNA鏈序列

    鏈名稱鏈序列 (5’–3’)
    AFAM–GTACACTGTAAAAAAAAAAAAAAACACTGTG–BHQ
    下載: 導(dǎo)出CSV

    表  3  汞、銀離子正交結(jié)果

    實(shí)驗(yàn)號A鏈濃度
    (A)    		離子濃度
    (B)    		反應(yīng)時(shí)間
    (C)    		F0–F
    1A1B1C11239.41042.8
    2A1B2C31572.91806.4
    3A1B3C21535.12010.4
    4A2B1C33243.21090.8
    5A2B2C26569.54773.2
    6A2B3C17675.07103.0
    7A3B1C23723.71201.6
    8A3B2C18459.99090.4
    9A3B3C311151.310298.2
    下載: 導(dǎo)出CSV
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出版歷程
  • 收稿日期:  2019-11-04
  • 修回日期:  2020-04-16
  • 網(wǎng)絡(luò)出版日期:  2020-05-12
  • 刊出日期:  2020-06-22

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