基于DNA適配體的熒光生物傳感器

董亞非; 胡文曉; 錢(qián)夢(mèng)瑤; 王越

doi:10.11999/JEIT190860

基于DNA適配體的熒光生物傳感器

doi: 10.11999/JEIT190860 cstr: 32379.14.JEIT190860

1.
陜西師范大學(xué)生命科學(xué)學(xué)院西安 710119
2.
陜西師范大學(xué)計(jì)算機(jī)科學(xué)學(xué)院西安 710119

基金項(xiàng)目: 國(guó)家自然科學(xué)基金(61572302)，陜西省自然科學(xué)基礎(chǔ)研究計(jì)劃(2020JM-298)

詳細(xì)信息

作者簡(jiǎn)介:
董亞非：男，1963年，教授，研究方向?yàn)镈NA計(jì)算和生物傳感器

胡文曉：女，1996年，碩士生，研究方向?yàn)镈NA計(jì)算和生物傳感器

錢(qián)夢(mèng)瑤：女，1995年，碩士生，研究方向?yàn)镈NA計(jì)算和生物傳感器

王越：女，1995年，碩士生，研究方向?yàn)镈NA計(jì)算和生物傳感器

通訊作者:
董亞非　dongyf@snnu.edu.cn

中圖分類(lèi)號(hào): TP212.3
計(jì)量
- 文章訪(fǎng)問(wèn)數(shù): 4472
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- PDF下載量: 307
- 被引次數(shù): 0
出版歷程
- 收稿日期: 2019-11-01
- 修回日期: 2020-03-14
- 網(wǎng)絡(luò)出版日期: 2020-04-03
- 刊出日期: 2020-06-22

DNA Aptamer-based Fluorescence Biosensor

1.
College of Life Science, Shaanxi Nomal University, Xi’an 710119, China
2.
School of Computer Science, Shaanxi Normal University, Xi’an 710119, China

Funds: The National Natural Science Foundation of China (61572302), The Natural Science Basic Research Plan in Shaanxi Province of China (2020JM-298)

摘要

摘要: 近年來(lái)，隨著DNA納米技術(shù)的飛速發(fā)展，基于DNA作為適配體的熒光生物傳感器不斷被大量學(xué)者研究和構(gòu)建，以實(shí)現(xiàn)對(duì)靶標(biāo)物質(zhì)的靈敏快速檢測(cè)。作為DNA納米技術(shù)的新興方向，基于DNA適配體的熒光生物傳感器具有巨大的應(yīng)用潛力。該文對(duì)近年來(lái)基于DNA適配體所構(gòu)建的熒光生物傳感器進(jìn)行了總結(jié)。包括熒光信號(hào)的實(shí)現(xiàn)：熒光染料標(biāo)記；非熒光染料標(biāo)記。熒光檢測(cè)信號(hào)的提升：酶介導(dǎo)的靶標(biāo)循環(huán)和信號(hào)擴(kuò)增策略；鏈置換反應(yīng)介導(dǎo)的靶標(biāo)循環(huán)和信號(hào)擴(kuò)增策略；基于鏈置換反應(yīng)和酶介導(dǎo)的靶標(biāo)循環(huán)和信號(hào)擴(kuò)增策略。在此基礎(chǔ)上對(duì)基于DNA適配體的熒光生物傳感器進(jìn)行展望并提出建議。
- 生物傳感器 /
- DNA適配體 /
- 熒光分析
Abstract: In recent years, with the rapid development of DNA nanotechnology, fluorescence biosensors based on DNA as aptamer are studied and constructed by a large number of scholars in order to realize the sensitive and rapid detection of target materials. As a new branch of DNA nanotechnology, fluorescence biosensors based on DNA aptamer have great application. The fluorescence biosensors based on DNA aptamers are summarized. The realization of fluorescence signal contains fluorescent dyes and non-fluorescent dye labeled. Enhancement of fluorescence signals involve enzyme-assisted, chain replacement reaction and both of all mediated target circulation and signals amplification strategy. On this basis, the fluorescence biosensor based on DNA aptamer is prospected and some suggestions are put forward.
- Biosensor /
- DNA aptamer /
- Fluorenscence analysis

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參考文獻(xiàn)(78)

樊春梅, 劉冬生. DNA納米技術(shù): 分子傳感、計(jì)算與機(jī)器[M]. 北京: 科學(xué)出版社, 2011: 65.

FAN Chunmei and LIU Dongsheng. DNA Nanotechnology: Molecular Sensing, Computing and Machines [M]. Beijing: Science Press, 2011: 65.

ELLINGTON A D and SZOSTAK J W. In vitro selection of RNA molecules that bind specific ligands[J]. Nature, 1990, 346(6287): 818–822. doi: 10.1038/346818a0

TUERK C and GOLD L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase[J]. Science, 1990, 249(4968): 505–510. doi: 10.1126/science.2200121

DHIMAN A, KALRA P, BANSAL V, et al. Aptamer-based point-of-care diagnostic platforms[J]. Sensors and Actuators B: Chemical, 2017, 246: 535–553. doi: 10.1016/j.snb.2017.02.060

SAPSFORD K E, BERTI L, and MEDINTZ I L. Materials for fluorescence resonance energy transfer analysis: beyond traditional donor-acceptor combinations[J]. Angewandte Chemie International Edition, 2006, 45(28): 4562–4589. doi: 10.1002/anie.200503873

MAZUMDER S, DEY R, MITRA M K, et al. Review: Biofunctionalized quantum dots in biology and medicine[J]. Journal of Nanomaterials, 2009: 38. doi: 10.1155/2009/815734

SUN Yali, FAN Jianfeng, CUI Linyan, et al. Fluorometric nanoprobes for simultaneous aptamer-based detection of carcinoembryonic antigen and prostate specific antigen[J]. Mikrochimica Acta, 2019, 186(3): 152. doi: 10.1007/s00604-019-3281-4

CHEN Xueqian, CHEN Shufan, HU Tianyu, et al. Fluorescent aptasensor for adenosine based on the use of quaternary CuInZnS quantum dots and gold nanoparticles[J]. Microchimica Acta, 2017, 184(5): 1361–1367. doi: 10.1007/s00604-017-2128-0

LU Yizhong and CHEN Wei. Sub-nanometre sized metal clusters: from synthetic challenges to the unique property discoveries[J]. Chemical Society Reviews, 2012, 41(9): 3594–3623. doi: 10.1039/c2cs15325d

ZHANG Manman, GAO Ge, DING Yalin, et al. A fluorescent aptasensor for the femtomolar detection of epidermal growth factor receptor-2 based on the proximity of G-rich sequences to Ag nanoclusters[J]. Talanta, 2019, 199: 238–243. doi: 10.1016/j.talanta.2019.02.014

LEE S T, RAHMAN R, MUTHOOSAMY K, et al. Amplification-free and direct fluorometric determination of telomerase activity in cell lysates using chimeric DNA-templated silver nanoclusters[J]. Microchimica Acta, 2019, 186(2): 81. doi: 10.1007/s00604-018-3194-7

KUNINGAS K, RANTANEN T, UKONAHO T, et al. Homogeneous assay technology based on upconverting phosphors[J]. Analytical Chemistry, 2005, 77(22): 7348–7355. doi: 10.1021/ac0510944

WANG Leyu, YAN Ruoxue, HUO Ziyang, et al. Fluorescence resonant energy transfer biosensor based on upconversion‐luminescent nanoparticles[J]. Angewandte Chemie International Edition, 2005, 44(37): 6054–6057. doi: 10.1002/anie.200501907

LI Hui, SUN Deen, LIU Yajie, et al. An ultrasensitive homogeneous aptasensor for kanamycin based on upconversion fluorescence resonance energy transfer[J]. Biosensors and Bioelectronics, 2014, 55: 149–156. doi: 10.1016/j.bios.2013.11.079

WANG Yujie, WEI Zikai, LUO Xianda, et al. An ultrasensitive homogeneous aptasensor for carcinoembryonic antigen based on upconversion fluorescence resonance energy transfer[J]. Talanta, 2019, 195: 33–39. doi: 10.1016/j.talanta.2018.11.011

HE Yue, LIN Yi, TANG Hongwu, et al. A graphene oxide-based fluorescent aptasensor for the turn-on detection of epithelial tumor marker mucin 1[J]. Nanoscale, 2012, 4(6): 2054–2059. doi: 10.1039/C2NR12061E

DOLATI S, RAMEZANI M, NABAVINIA M S, et al. Selection of specific aptamer against enrofloxacin and fabrication of graphene oxide based label-free fluorescent assay[J]. Analytical Biochemistry, 2018, 549: 124–129. doi: 10.1016/j.ab.2018.03.021

ZHAO Lianjing, CHENG Ming, LIU Guannan, et al. A fluorescent biosensor based on molybdenum disulfide nanosheets and protein aptamer for sensitive detection of carcinoembryonic antigen[J]. Sensors and Actuators B: Chemical, 2018, 273: 185–190. doi: 10.1016/j.snb.2018.06.004

CHEN Feng, LIU Yi, CHEN Chunyan, et al. Respective and simultaneous detection tumor markers CA125 and STIP1 using aptamer-based fluorescent and RLS sensors[J]. Sensors and Actuators B: Chemical, 2017, 245: 470–476. doi: 10.1016/j.snb.2017.01.155

YI Haoyang, YAN Zhiyu, WANG Lumei, et al. Fluorometric determination for ofloxacin by using an aptamer and SYBR Green I[J]. Microchimica Acta, 2019, 186(10): 668. doi: 10.1007/s00604-019-3788-8

BAHREYNI A, TAHMASEBI S, RAMEZANI M, et al. A novel fluorescent aptasensor for sensitive detection of PDGF-BB protein based on a split complementary strand of aptamer and magnetic beads[J]. Sensors and Actuators B: Chemical, 2019, 280: 10–15. doi: 10.1016/j.snb.2018.10.047

LIN Sheng, HE Bingyong, YANG Chao, et al. Luminescence switch-on assay of interferon-gamma using a G-quadruplex-selective iridium(III) complex[J]. Chemical communications, 2015, 51(89): 16033–16036. doi: 10.1039/C5CC06655G

CHEN Mingjian, MA Changbei, YAN Ying, et al. A label-free fluorescence method based on terminal deoxynucleotidyl transferase and thioflavin T for detecting prostate-specific antigen[J]. Analytical and Bioanalytical Chemistry, 2019, 411(22): 5779–5784. doi: 10.1007/s00216-019-01958-0

WEI Yulian, ZHOU Wenjiao, LIU Jun, et al. Label-free and homogeneous aptamer proximity binding assay for fluorescent detection of protein biomarkers in human serum[J]. Talanta, 2015, 141: 230–234. doi: 10.1016/j.talanta.2015.04.005

TANG Xiaomin, LI Xiaotong, MA D L, et al. A label-free triplex-to-G-qadruplex molecular switch for sensitive fluorescent detection of acetamiprid[J]. Talanta, 2018, 189: 599–605. doi: 10.1016/j.talanta.2018.07.025

GUO Limin and ZHAO Qiang. Determination of the platelet-derived growth factor BB by a competitive thrombin-linked aptamer-based Fluorometric assay[J]. Microchimica Acta, 2016, 183(12): 3229–3235. doi: 10.1007/s00604-016-1978-1

ALI M M, LI Feng, ZHANG Zhiqing, et al. Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine[J]. Chemical Society Reviews, 2018, 43(10): 3324–3341. doi: 10.1039/C3CS60439J

LI Lu, WANG Qian, FENG Jie, et al. Highly sensitive and homogeneous detection of membrane protein on a single living cell by aptamer and nicking enzyme assisted signal amplification based on microfluidic droplets[J]. ACS Publications, 2014, 86(10): 5101–5107. doi: 10.1021/ac500881p

ZHANG Zhonghui, ZHANG Feng, HE Peng, et al. Fluorometric determination of mercury(II) by using thymine-thymine mismatches as recognition elements, toehold binding, and enzyme-assisted signal amplification[J]. Microchimica Acta, 2019, 186(8): 1–6. doi: 10.1007/s00604-019-3683-3

ZHANG Zhenzhu and ZHANG Chunyang. Highly sensitive detection of protein with aptamer-based target-triggering two-stage amplification[J]. ACS Publications, 2012, 84(3): 1623–1629. doi: 10.1021/ac2029002

ZHEN Zhen, LIU Jinwen, QIAN Wen, et al. Homogeneous label-free protein binding assay using small-molecule-labeled DNA nanomachine with DNAzyme-Based chemiluminescence detection[J]. Talanta, 2020, 206: 120175. doi: 10.1016/j.talanta.2019.120175

HUANG Ru, LIAO Yuhui, ZHOU Xiaoming, et al. Toehold-mediated nonenzymatic amplification circuit on graphene oxide fluorescence switching platform for sensitive and homogeneous microRNA detection[J]. Analytica Chimica Acta, 2015, 888: 162–172. doi: 10.1016/j.aca.2015.07.041

WANG Xiuzhong, JIANG Aiwen, HOU Ting, et al. Enzyme-free and label-free fluorescence aptasensing strategy for highly sensitive detection of protein based on target-triggered hybridization chain reaction amplification[J]. Biosensors and Bioelectronics, 2015, 70: 324–329. doi: 10.1016/j.bios.2015.03.053

HU Jiaming, SHENG Yan, KWAK K J, et al. A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection[J]. Nature Communications, 2017, 8(1): 1683. doi: 10.1038/s41467-017-01942-1

ZHANG Xiaobing, ZHANG Zidong, XING Hang, et al. Catalytic and molecular beacons for amplified detection of metal ions and organic molecules with high sensitivity[J]. ACS Publications, 2010, 82(12): 5005–5011. doi: 10.1021/ac1009047

SATO S, FUJITA K, KANAZAWA M, et al. Electrochemical assay for deoxyribonuclease I activity[J]. Analytical Biochemistry, 2008, 381(2): 233–239. doi: 10.1016/j.ab.2008.07.014

ZHANG Jun, RAN Fengying, ZHOU Wenbo, et al. Ultrasensitive fluorescent aptasensor for MUC1 detection based on deoxyribonuclease I-aided target recycling signal amplification[J]. RSC Advances, 2018, 8(56): 32009–32015. doi: 10.1039/C8RA06498A

WANG Hui, CHEN Hui, HUANG Zhipeng, et al. DNase I enzyme-aided fluorescence signal amplification based on graphene oxide-DNA aptamer interactions for colorectal cancer exosome detection[J]. Talanta, 2018, 184: 219–226. doi: 10.1016/j.talanta.2018.02.083

CHAN S H, ZHU Zhenyu, VAN ETTEN J L, et al. Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification[J]. Nucleic Acids Research, 2004, 32(21): 6187–6199. doi: 10.1093/nar/gkh958

LI Xiang, DING Xuelian and FAN Jing. Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform[J]. Analyst, 2015, 140(23): 7918–7925. doi: 10.1039/c5an01759a

NING Yi, HU Jue, WEI Ke, et al. Fluorometric determination of mercury(II) via a graphene oxide-based assay using exonuclease III-assisted signal amplification and thymidine-Hg(II)-thymidine interaction[J]. Microchimica Acta, 2019, 186(4): 216. doi: 10.1007/s00604-019-3332-x

XIAO Xue, TAO Jing, ZHANG Hongzhi, et al. Exonuclease III-assisted graphene oxide amplified fluorescence anisotropy strategy for ricin detection[J]. Biosensors and Bioelectronics, 2016, 85: 822–827. doi: 10.1016/j.bios.2016.05.091

CUI Miao, XIAO Xianjin, ZHAO Meiping, et al. Detection of single nucleotide polymorphism by measuring extension kinetics with T7 exonuclease mediated isothermal amplification[J]. Analyst, 2018, 143(1): 116–122. doi: 10.1039/C7AN00875A

JACOBSEN H, KLENOW H, and OVERGAARD-HANSEN K. The N-terminal amino-acid sequences of DNA polymerase I from Escherichia coli and of the large and the small fragments obtained by a limited proteolysis[J]. European Journal of Biochemistry, 1974, 45(2): 623–627. doi: 10.1111/j.1432-1033.1974.tb03588.x

DERBYSHIRE V, FREEMONT P S, SANDERSON M R, et al. Genetic and crystallographic studies of the 3’, 5’-exonucleolytic site of DNA polymerase I[J]. Science, 1988, 240(4849): 199–201. doi: 10.1126/science.2832946

ZHANG D Y and SEELIG G. Dynamic DNA nanotechnology using strand-displacement reactions[J]. Nature Chemistry, 2011, 3(2): 103–113. doi: 10.1038/nchem.957

DIRKS R M and PIERCE N A. Triggered amplification by hybridization chain reaction[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(43): 15275–15278. doi: 10.1073/pnas.0407024101

LI Junlong, SUN Kexin, CHEN Zhongping, et al. A fluorescence biosensor for VEGF detection based on DNA assembly structure switching and isothermal amplification[J]. Biosensors and Bioelectronics, 2017, 89: 964–969. doi: 10.1016/j.bios.2016.09.078

ZHOU Qingzhen, YAN Hongxia, RAN Fengying, et al. Ultrasensitive enzyme-free fluorescent detection of VEGF165 based on target-triggered hybridization chain reaction amplification[J]. RSC Advances, 2018, 8(45): 25955–25960. doi: 10.1039/C8RA04721A

LI Qiong, LIU Zhi, ZHOU Danhua, et al. A cascade toehold-mediated strand displacement strategy for label-free and sensitive non-enzymatic recycling amplification detection of the HIV-1 gene[J]. Analyst, 2019, 144(6): 2173–2178. doi: 10.1039/C8AN02340A

YIN Peng, CHOI H M T, CALVERT C R, et al. Programming biomolecular self-assembly pathways[J]. Nature, 2008, 451(7176): 318–322. doi: 10.1038/nature06451

XU Jiayao, SHI Ming, HUANG Huakui, et al. A fluorescent aptasensor based on single oligonucleotide-mediated isothermal quadratic amplification and graphene oxide fluorescence quenching for ultrasensitive protein detection[J]. Analyst, 2018, 143(16): 3918–3925. doi: 10.1039/c8an01032c

ZHOU Jie, MENG Lingchang, YE Weiran, et al. A sensitive detection assay based on signal amplification technology for Alzheimer’s disease’s early biomarker in exosome[J]. Analytica Chimica Acta, 2018, 1022: 124–130. doi: 10.1016/j.aca.2018.03.016

YANG Wenting, ZHOU Xingxing, ZHAO Jianmin, et al. A cascade amplification strategy of catalytic hairpin assembly and hybridization chain reaction for the sensitive fluorescent assay of the model protein carcinoembryonic antigen[J]. Microchimica Acta, 2018, 185(2): 100. doi: 10.1007/s00604-017-2620-6

ZHANG Zheng, HAN Jialun, LI Yitan, et al. A sensitive and recyclable fluorescence aptasensor for detection and extraction of platelet-derived growth factor BB[J]. Sensors and Actuators B: Chemical, 2018, 277: 179–185. doi: 10.1016/j.snb.2018.09.013

HU Kun, LIU Jinwen, CHEN Jia, et al. An amplified graphene oxide-based fluorescence aptasensor based on target-triggered aptamer hairpin switch and strand-displacement polymerization recycling forbioassays[J]. Biosensors and Bioelectronics, 2013, 42: 598–602. doi: 10.1016/j.bios.2012.11.025

LI Chunhong, XIAO Xue, TAO Jing, et al. A graphene oxide-based strand displacement amplification platform for ricin detection using aptamer as recognition element[J]. Biosensors and Bioelectronics, 2017, 91: 149–154. doi: 10.1016/j.bios.2016.12.010

HE Jinglin, ZHANG Yang, YANG Chan, et al. Hybridization chain reaction based DNAzyme fluorescent sensor for _L-histidine assay[J]. Analytical Methods, 2019, 11(16): 2204–2210. doi: 10.1039/C9AY00526A

YIN Jinjin, LIU Yaqing, WANG Shuo, et al. Engineering a universal and label-free evaluation method for mycotoxins detection based on strand displacement amplification and G-quadruplex signal amplification[J]. Sensors and Actuators B: Chemical, 2018, 256: 573–579. doi: 10.1016/j.snb.2017.10.083

CHEN Piaopiao, HUANG Ke, ZHANG Peng, et al. Exonuclease III-assisted strand displacement reaction-driven cyclic generation of G-quadruplex strategy for homogeneous fluorescent detection of melamine[J]. Talanta, 2019, 203: 255–260. doi: 10.1016/j.talanta.2019.05.020

DARWISH I A, WANI T A, KHALIL N Y, et al. Novel automated flow-based immunosensor for real-time measurement of the breast cancer biomarker CA15–3 in serum[J]. Talanta, 2012, 97: 499–504. doi: 10.1016/j.talanta.2012.05.005

LOISEAU A, ZHANG Lu, HU D, et al. Core-shell gold/silver nanoparticles for localized surface plasmon resonance-based naked-eye toxin biosensing[J]. ACS Applied Materials & Interfaces, 2019, 11(50): 46462–46471. doi: 10.1021/acsami.9b14980

CHUANG C S, WU C Y, JUAN P H, et al. LMP1 gene detection using a capped gold nanowire array surface plasmon resonance sensor in a microfluidic chip[J]. Analyst, 2020, 145(1): 52–60. doi: 10.1039/c9an01419e

GHODAKE G, SHINDE S, SARATALE R G, et al. Silver nanoparticle probe for colorimetric detection of aminoglycoside antibiotics: picomolar-level sensitivity toward streptomycin in water, serum, and milk samples[J]. Journal of the Science of Food and Agriculture, 2020, 100(2): 874–884. doi: 10.1002/jsfa.10129

ZHAO Lifang, WEI Qin, WU Hua, et al. Ionic liquid functionalized graphene based immunosensor for sensitive detection of carbohydrate antigen 15–3 integrated with Cd²⁺-functionalized nanoporous TiO₂ as labels[J]. Biosensors and Bioelectronics, 2014, 59: 75–80. doi: 10.1016/j.bios.2014.03.006

JIANG Xinya, WANG Haijun, YUAN Ruo, et al. Sensitive electrochemiluminescence detection for CA15–3 based on immobilizing luminol on dendrimer functionalized ZnO nanorods[J]. Biosensors and Bioelectronics, 2015, 63: 33–38. doi: 10.1016/j.bios.2014.07.009

HAMD-GHADAREH S, SALIMI A, PARSA S, et al. Simultaneous biosensing of CA125 and CA15–3 tumor markers and imaging of OVCAR-3 and MCF-7 cells lines via bi-color FRET phenomenon using dual blue-green luminescent carbon dots with single excitation wavelength[J]. International Journal of Biological Macromolecules, 2018, 118: 617–628. doi: 10.1016/j.ijbiomac.2018.06.116

LU Zijing, WANG Peng, XIONG Weiwei, et al. Simultaneous detection of mercury (II), lead (II) and silver (I) based on fluorescently labelled aptamer probes and graphene oxide[J]. Environmental Technology, 2020, 317: 1–27. doi: 10.1080/09593330.2020.1721565

田威, 黃高明. 非理想關(guān)聯(lián)下多傳感器系統(tǒng)誤差的穩(wěn)健估計(jì)[J]. 電子與信息學(xué)報(bào), 2018, 40(3): 641–647. doi: 10.11999/JEIT170579

TIAN Wei and HUANG Gaoming. Robust multisensor bias estimation under nonideal association[J]. Journal of Electronics &Information Technology, 2018, 40(3): 641–647. doi: 10.11999/JEIT170579

YANG Jing, DONG Chen, DONG Yafei, et al. Logic nanoparticle beacon triggered by the binding-induced effect of multiple inputs[J]. ACS Applied Materials & Interfaces, 2014, 6(16): 14486–14492. doi: 10.1021/am5036994

YANG Jing, JIANG Shuoxing, LIU Xiangrong, et al. Aptamer-binding directed DNA origami pattern for logic gates[J]. ACS Applied Materials & Interfaces, 2016, 8(49): 34054–34060. doi: 10.1021/acsami.6b10266

PAN Linqiang, WANG Zhiyu, LI Yifan, et al. Nicking enzyme-controlled toehold regulation for DNA logic circuits[J]. Nanoscale, 2017, 9(46): 18223–18228. doi: 10.1039/C7NR06484E

YANG Jing, WU Ranfeng, LI Yifan, et al. Entropy-driven DNA logic circuits regulated by DNAzyme[J]. Nucleic Acids Research, 2018, 46(16): 8532–8541. doi: 10.1093/nar/gky663

劉素艷, 劉元安, 吳帆, 等. 物聯(lián)網(wǎng)中基于相似性計(jì)算的傳感器搜索[J]. 電子與信息學(xué)報(bào), 2018, 40(12): 3020–3027. doi: 10.11999/JEIT171085

LIU Suyan, LIU Yuanan, WU Fan, et al. Sensor search based on sensor similarity computing in the internet of things[J]. Journal of Electronics &Information Technology, 2018, 40(12): 3020–3027. doi: 10.11999/JEIT171085

RANALLO S, PRéVOST-TREMBLAY C, IDILI A, et al. Antibody-powered nucleic acid release using a DNA-based nanomachine[J]. Nature Communications, 2017, 8: 15150. doi: 10.1038/ncomms15150

沈賀, 張立明, 張智軍. 石墨烯在生物醫(yī)學(xué)領(lǐng)域的應(yīng)用[J]. 東南大學(xué)學(xué)報(bào)(醫(yī)學(xué)版), 2011, 30(1): 218–223. doi: 10.3969/j.issn.1671-6264.2011.01.035

SHEN He, ZHANG Liming, and ZHANG Zhijun. Graphene for biomedical applications[J]. Journal of Southeast University (Medical Science Edition), 2011, 30(1): 218–223. doi: 10.3969/j.issn.1671-6264.2011.01.035

LIU Zhuang, ROBINSON J T, SUN Xiaoming, et al. PEGylated nanographene oxide for delivery of water-insoluble cancer drugs[J]. Journal of the American Chemical Society, 2008, 130(33): 10876–10877. doi: 10.1021/ja803688x

XU Fei, WU Tingfang, SHI Xiaolong, et al. A study on a special DNA nanotube assembled from two single-stranded tiles[J]. Nanotechnology, 2019, 30(11): 115602. doi: 10.1088/1361-6528/aaf9bc

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